UBR4 deficiency causes male sterility and testis abnormal in Drosophila

Introduction It has been established that UBR4 encodes E3 ubiquitin ligase, which determines the specificity of substrate binding during protein ubiquitination and associated with various functions nervous system but not reproductive system. Herein, we explored role on fertility a Drosophila model . Methods Different Ubr4 knockdown flies were using UAS/GAL4 activating sequence Fertility, hatchability, testis morphology studied, bioinformatics analyses conducted. Our results indicated deficiency could induce male sterility influent egg hatchability in Results We found affected morphological analysis. Proteomics analysis 188 upregulated proteins 175 downregulated flies. Gene Ontology revealed significant upregulation CG11598 Sfp65A , downregulation Pelota These involved biometabolic or process regulated are important generation sperm storage promotion. Bioinformatics verified was low expressed cryptorchidism patients, further supported fertility. Discussion Overall, our findings suggest promote infertility may be modification by upregulating whereas downregulating expression.